This page is for submission of requests for using the Institution’s Multiphoton Imaging facility in the Information Processing Lab (IPL).
Kindly go through all instructions for use requests of the multi-photon imaging setup. Overall components of the setup are also provided below. Once you send a request depending on availability and your required duration a time slot will be made available. Training was done by Bruker earlier and only persons having attended the training will be allowed. Otherwise you will need to speak with persons who went through the training and be accompanied by one such person.
You may please see here and here for some basic information on 2-photon microscopy.
Instructions for submission:
Please submit your request through the form provided below. Please provide the following information:
1) Name of PI/Supervisor:
2) Email of PI/Supervisor:
3) Name of person doing the experiment:
4) Whether received training or not:
5) If student/fellow: Level (Doctoral/Btech/Mtech/BSc/MSc/JRF/SRF/RA)
6) Description of experiment:
(Please provide details and why this imaging setup is necessary – please understand that if your experiment can be done on a confocal or live cell imaging setup you will be better off doing it there)
7) Please state if you need to discuss the experiment or the problem is not fully defined and need to refine it (in that case, please also request your supervisor to email us).
For any questions please email us at sharba AT ece dot iitkgp dot ernet dot in
Kindly go through all instructions for use requests of the multi-photon imaging setup. Overall components of the setup are also provided below. Once you send a request depending on availability and your required duration a time slot will be made available. Training was done by Bruker earlier and only persons having attended the training will be allowed. Otherwise you will need to speak with persons who went through the training and be accompanied by one such person.
You may please see here and here for some basic information on 2-photon microscopy.
Instructions for submission:
Please submit your request through the form provided below. Please provide the following information:
1) Name of PI/Supervisor:
2) Email of PI/Supervisor:
3) Name of person doing the experiment:
4) Whether received training or not:
5) If student/fellow: Level (Doctoral/Btech/Mtech/BSc/MSc/JRF/SRF/RA)
6) Description of experiment:
(Please provide details and why this imaging setup is necessary – please understand that if your experiment can be done on a confocal or live cell imaging setup you will be better off doing it there)
7) Please state if you need to discuss the experiment or the problem is not fully defined and need to refine it (in that case, please also request your supervisor to email us).
For any questions please email us at sharba AT ece dot iitkgp dot ernet dot in
Setup:
The multiphoton imaging setup presently available with us has the following:
Imaging Laser: Spectra-Physics Insight
Uncaging/Photostimulation: Mai Tai Deep See eHP (Unacaging optics and functional mapping system present). This laser can also be used in conjunction with the imaging laser for dual wavelength imaging.
Lasers for Optogenetic Applications: 473 nm and 563 nm (for Channel-rhodopsin and Halo-rhodopsin activation) – Coupling with single mode fiber is possible
Detectors: GaAsP
Filters: Green and Red
Epifluorescence imaging setup is also included.
This microscope is for in-vivo applications and hence there is no stage available, with the region beneath the objective being completely empty. Please bring your own stage if you have one or email us to discuss. A custom built stage to hold animals (mice) to image from the brain of the intact animal is present.
For further details on the setup/capabilities please email us.
The multiphoton imaging setup presently available with us has the following:
Imaging Laser: Spectra-Physics Insight
Uncaging/Photostimulation: Mai Tai Deep See eHP (Unacaging optics and functional mapping system present). This laser can also be used in conjunction with the imaging laser for dual wavelength imaging.
Lasers for Optogenetic Applications: 473 nm and 563 nm (for Channel-rhodopsin and Halo-rhodopsin activation) – Coupling with single mode fiber is possible
Detectors: GaAsP
Filters: Green and Red
Epifluorescence imaging setup is also included.
This microscope is for in-vivo applications and hence there is no stage available, with the region beneath the objective being completely empty. Please bring your own stage if you have one or email us to discuss. A custom built stage to hold animals (mice) to image from the brain of the intact animal is present.
For further details on the setup/capabilities please email us.